ANTI INFLAMMATORY ACTIVITY OF VITEX NEGUNDO PDF

J Ethnopharmacol. Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. This study confirmed the oral anti-inflammatory, analgesic and antihistamine properties of mature fresh leaves MFL of Vitex negundo L. Verbenaceae claimed in the Ayurveda medicine by orally treating a water extract of the leaves to rats. In the formaldehyde-induced rat paw oedema test, the 2.

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Department of Pharmacognosy and Phytochemistry, Principal K. This article has been cited by other articles in PMC. Abstract Reactive oxygen species are implicated in various inflammatory disorders. Vitex negundo is mentioned in Ayurveda as useful in treating arthritic disorders. The present work was undertaken to evaluate the antioxidant potential and anti-inflammatory activity of the plant. The total methanol extract of the plant was standardized in terms of total polyphenols.

The extract also exhibited a strong free radical scavenging activity by 1,1-diphenylpicrylhydrazyl method and caused a significant reduction in the formation of thiobarbituric acid reacting substances when evaluated for its lipid peroxidation inhibitory activity. The results strongly suggest that radical quenching may be one of the mechanisms responsible for its antiinflammatory activity.

Keywords: Antiinflammatory activity, antioxidant activity, total polyphenol content, Vitex negundo Ayurveda mentions several plants acting as antiinflammatory agents in arthritic disorders.

The tissue damage then provokes inflammatory response by the production of mediators and chemotactic factors 4. The reactive oxygen species are also known to activate matrix metalloproteinase e. Hence the agents that can scavenge these reactive oxygen species can be beneficial in the treatment of such inflammatory disorders.

The easy availability of this plant, coupled with its reported usefulness in arthritic disorders prompted us to further explore its antiinflammatory and antioxidant activity. The plant material was collected from Versova, Mumbai and plant sample was authenticated from Blatter Herbarium, St. A herbarium specimen of this plant has been deposited at Principal K.

Kundnani College of Pharmacy. Gum acacia, phosphoric acid, potassium ferricyanide, ferric chloride were of LR grade and obtained from local suppliers. Gallic acid and 1,1-diphenylpicrylhydrazyl was procured from Sigma Aldrich Co. Louis, Mo. Ferrous sulphate, ascorbic acid, sodium chloride, potassium chloride, all of LR grade were obtained from local suppliers.

Diclofenac sodium was obtained from Mac Labs, Mumbai as a gift sample. The extract was concentrated under vacuum. The total polyphenolic content of the whole percolate extract was determined using Prussian blue method 6.

The content were mixed well and kept at room temperature for 15 min. The total polyphenols were estimated as gallic acid equivalents. This method is based on reduction in absorbance of a methanol solution of coloured free radical, 1,1-diphynylpicrylhydrazyl 7. Ascorbic acid was used as positive control. During the aerobic incubation of tissue homogenates, malondialdehydes are formed which on reaction with thiobarbituric acid produced a pink colour. The pink colour complex of thiobarbituric acid reacting substances was monitored at nm 8 — Curcumin was used as positive control.

Mice were fasted overnight before the day of experiment and the liver was promptly excised after sacrificing the mice. The liver was weighed and perfused with 0. After washing, tissue homogenate was prepared in 10 volumes of ice-cold potassium chloride by homogenizing for 15 min.

The reaction mixture contained 0. To this reaction mixture thiobarbituric acid was added and the mixture was boiled for 15 min. The tubes were then centrifuged at rpm for 10 min and cooled.

Absorbance of the resulting pink chromogen was recorded at nm. The antiinflammatory activity was carried out on Wistar rats using the carrageenan-induced rat paw method 11 , Necessary permission was obtained from the Institutional Animal Ethics Committee.

Suspension of diclofenac sodium 6. Edema in one of the paws of the rat was induced by injecting into plantar aponeurosis 0. All vehicles, standard, and the test compound were fed to the rats one hour before the injection. Volume of the injected paw was measured plethysmographically immediately after the injection and then again at hourly intervals up to 4h. The whole percolate extract of V. Hence the extract was standardized in terms of total polyphenol content and found to contain The phenolics, particularly polyphenols exhibit a wide variety of beneficial activities in mammals including antiviral, antibacterial, immune stimulating, antiallergic, antihypertensive, antiischemic, antiarrythmic, antithrombotic, hypocholesteromic, hepatoprotective, antiinflammatory, anticarcinogenic Flavonoids are an important group of polyphenols and are reported to inhibit prostaglandin synthesis, which are known mediators of inflammation When it was evaluated for its antioxidant activity by studying its lipid peroxidation inhibitory activity; it exhibited a concentration dependent inhibition of formation of thiobarbituric acid reacting substances Table 1 with a potent antioxidant activity.

Lipid peroxidation is responsible for damaging cell membranes thereby further intensifying inflammatory damage. A positive lipid peroxidation inhibitory activity of the extract of V. TABLE 1.

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Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo.

Department of Pharmacognosy and Phytochemistry, Principal K. This article has been cited by other articles in PMC. Abstract Reactive oxygen species are implicated in various inflammatory disorders. Vitex negundo is mentioned in Ayurveda as useful in treating arthritic disorders. The present work was undertaken to evaluate the antioxidant potential and anti-inflammatory activity of the plant. The total methanol extract of the plant was standardized in terms of total polyphenols.

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